Dengue virus type 2 NS3 protease and NS2B-NS3 protease precursor induce apoptosis
نویسندگان
چکیده
منابع مشابه
Dengue Virus NS2B/NS3 Protease Inhibitors Exploiting the Prime Side.
The mosquito-transmitted dengue virus (DENV) infects millions of people in tropical and subtropical regions. Maturation of DENV particles requires proper cleavage of the viral polyprotein, including processing of 8 of the 13 substrate cleavage sites by dengue virus NS2B/NS3 protease. With no available direct-acting antiviral targeting DENV, NS2/NS3 protease is a promising target for inhibitor d...
متن کاملIn vitro determination of dengue virus type 2 NS2B-NS3 protease activity with fluorescent peptide substrates.
The NS2B-NS3(pro) polyprotein segment from the dengue virus serotype 2 strain 16681 was purified from overexpressing E. coli by metal chelate affinity chromatography and gel filtration. Enzymatic activity of the refolded NS2B-NS3(pro) protease complex was determined in vitro with dansyl-labeled peptide substrates, based upon native dengue virus type 2 cleavage sites. The 12mer substrate peptide...
متن کاملFlavonoid from Carica papaya inhibits NS2B-NS3 protease and prevents Dengue 2 viral assembly
UNLABELLED Dengue virus belongs to the virus family Flaviviridae. Dengue hemorrhagic disease caused by dengue virus is a public health problem worldwide. The viral non structural 2B and 3 (NS2B-NS3) protease complex is crucial for virus replication and hence, it is considered to be a good anti-viral target. Leaf extracts from Carica papaya is generally prescribed for patients with dengue fever,...
متن کاملInhibition of the NS2B-NS3 Protease – Towards aCausative Therapy for Dengue Virus Diseases: Chapter 08
The high impact of diseases caused by dengue viruses on global health is now reflected in an increased interest in the identification of drug targets and the rationale-based development of antiviral inhibitors which are suitable for a causative treatment of severe forms of dengue virus infections – dengue haemorrhagic fever and dengue shock syndrome. A promising target for the design of specifi...
متن کاملNovel dengue virus-specific NS2B/NS3 protease inhibitor, BP2109, discovered by a high-throughput screening assay.
Dengue virus (DENV) causes disease globally, with an estimated 25 to 100 million new infections per year. At present, no effective vaccine is available, and treatment is supportive. In this study, we identified BP2109, a potent and selective small-molecule inhibitor of the DENV NS2B/NS3 protease, by a high-throughput screening assay using a recombinant protease complex consisting of the central...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2003
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.19022-0